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KMID : 0900920000240040419
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Korean journal of Animal Reproduction
2000 Volume.24 No. 4 p.419 ~ p.428
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Expression of Antisense Mouse Obese Gene in Transgenic Mice
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Kwon B.-S.
Hong K.-H.
Jahng J.-W.
Lee H.-T.
Chung K.-S.
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Abstract
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Leptin, the product of obese (ob) gene, is an adipocyte-derived satiety factor that plays a major role in the regulation of food intake, energy homeostasis, body weight, reproductive physiology and neuropeptide secretion. The present study was designed to generate transgenic mice expressing antisense mouse ob (mob) gene. Total RNA was extracted from the adipose tissues of mouse, then reverse transcription was performed. The 303 and 635 bp fragments of anti I and II cDNAs were amplified from mob cDNAs by PCR. The two mob cDNAs were reversely ligated into between adipose tissue specific aP2 promote and SV40 poly(A) site. Transgenic mice carrying two different kinds of antisense mob transgenes were generated by DNA microinjection into pronucleus. Total 14 transgenic mice were born, and the 4 and 5 founder lines of the transgenic mice with anti I and II transgenes were respectively established. Antisense mRNA expression was detected in transgenic F_1 mice by RT-PCR analysis. This result suggests that the transgenic mice expressing antisense mob mRNA may be useful as an animal disease model to be obesity caused by decreased amount of leptin secretion.
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KEYWORD
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Leptin, Transgenic, Antisense, aP2 promoter, Obesity
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